Trimer Primer Synthesis
The development of the chemical synthesis method of the primer laid the foundation for the predictable engineering transformation of the biological system, promoted the development of artificial DNA synthesis technology, saved costs for the construction and screening of the polypeptide antibody library. The simplified primer of a single nucleoside synthesis will inevitably produce redundant mutations and termination codons at the mutation site. Based on Trimer's hybrid element design, the amino acids that can ensure that the amino acids of the mutant position are composed of the proportion of the design, meet the diversity and preferences of amino acids at the specific positioning point, and ensure the diversity of downstream experiments and the convenience of screening.
Using Trimer phosphoramidites to synthesize nucleotide sequences containing specific points of mutation, mutant site can cover 20 amino acid distribution. Multiple Trimer primers are used as the overall synthetic raw material and synthesize to the predetermined position in the sequence to obtain a primer library that meets the predetermined sequence. In addition, the Trimer primer can also be used in the construction of downstream combination of mutation library, supporting the study of subsequent protein orientation evolution, antibody screening, drug target screening and drug discovery, enzyme optimization.
Figure 1
Trimer primer advantages：
1. Meet the diversity and preferences of the specific positioning point amino acid.
2. Avoid redundant mutations and termination codons.
3. Trimer primer can accurately control the proportion of amino acids of mutation position.
Empowering the thriving development of peptide therapeutics worldwide, enabling cost‑effective access to peptide medicines for patients around the globe, and contributing to human health.
Hunan Zhongsheng Quanpeptide Biotechnology Co., Ltd.
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